| 质粒名称: | pEGFP-C1-VEEV nsP3 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002851 |
| 产品用途: | Mammalian expression of mEGFP-VEEV nsP3 |
| 存储实验室: | Peiguo YANG Lab |
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载体骨架基本信息 |
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| 载体名称: | pEGFP-C1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
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插入基因信息 |
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| 插入基因名称: | VEEV nsP3 |
| 物种来源: | Alphavirus |
| 密码子优化: | 大肠杆菌表达优化 |
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文献引用方法 |
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| 参考文献部分: |
Alphaviral non-structural protein-host RBP co-condensation as a mechanism to sustain virus replication
Molecular Cell, Volume 86, Issue 9, 2026
Yi Liu, Peiguo Yang
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pEGFP-C1-VEEV nsP3
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pEGFP-C1-VEEV nsP3载体质粒基本信息
pEGFP-C1-VEEV nsP3质粒图谱载体图谱和pEGFP-C1-VEEV nsP3载体序列质粒序列多克隆位点信息
pEGFP-C1-VEEV nsP3质粒载体简介
pEGFP-C1-VEEV nsP3质粒序列载体序列
LOCUS pEGFP-C1-VEEV nsP3 6352 bp DNA circular SYN 16-JUL-2026 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6352) AUTHORS 1243235 TITLE Direct Submission JOURNAL Exported Sep 7, 2026 from SnapGene 6.0.2 https://www.biofeng.com FEATURES Location/Qualifiers source 1..6352 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 304..507 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" CDS 552..1268 /codon_start=1 /product="enhanced GFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mEGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1274..1298) /label=p61-1155 primer_bind 1284..1322 /label=p61-1152 CDS 1299..2951 /codon_start=1 /label=VEEV nsP3 TC83 (E.coli codon) /translation="APSYHVVRGDIATATEGVIINAANSKGQPGGGVCGALYKKFPESF DLQPIEVGKARLVKGAAKHIIHAVGPNFNKVSEVEGDKQLAEAYESIAKIVNDNNYKSV AIPLLSTGIFSGNKDRLTQSLNHLLTALDTTDADVAIYCRDKKWEMTLKEAVARREAVE EICISDDSSVTEPDAELVRVHPKSSLAGRKGYSTSDGKTFSYLEGTKFHQAAKDIAEIN AMWPVATEANEQVCMYILGESMSSIRSKCPVEESEASTPPSTLPCLCIHAMTPERVQRL KASRPEQITVCSSFPLPKYRITGVQKIQCSQPILFSPKVPAYIHPRKYLVETPPVDETP EPSAENQSTEGTPEQPPLITEDETRTRTPEPIIIEEEEEDSISLLSDGPTHQVLQVEAD IHGPPSVSSSSWSIPHASDFDVDSLSILDTLEGASVTSGATSAETNSYFAKSMEFLARP VPAPRTVFRNPPHPAPRTRTPSLAPSRACSRTSLVSTPPGVNRVITREELEALTPSRTP SRSVSRTSLVSNPPGVNRVITREEFEAFVAQQQ" primer_bind complement(2261..2308) /label=p61-1411 misc_feature 2289..2951 /label=HVD misc_feature 2289..2471 /label=Fragment A primer_bind 2289..2312 /label=p61-1412 misc_feature 2472..2690 /label=Fragment B misc_feature 2691..2951 /label=Fragment C misc_feature 2730..2828 /label=FXR binding motif misc_feature 2832..2930 /label=FXR binding motif primer_bind complement(2931..2966) /label=p61-1153 primer_bind 2952..2976 /label=p61-1154 polyA_signal 3081..3202 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(3209..3664) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3691..3795 /gene="bla" /label=AmpR promoter promoter 3797..4154 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4005..4140 /label=SV40 ori /note="SV40 origin of replication" CDS 4189..4983 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 5215..5262 /label=HSV TK poly(A) signal /note="herpesvirus thymidine kinase polyadenylation signal" rep_origin 5591..6179 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" enhancer join(6352,1..303) /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 481 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 541 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 601 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 661 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 721 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 781 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 841 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 901 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 961 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 1021 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 1081 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 1141 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg 1201 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 1261 tgtacaagtc cggactcaga tctcgagctc aagcttccgc tcctagttat catgttgttc 1321 gtggtgacat tgcaaccgca accgaaggcg tgattattaa tgcagccaat agtaaaggtc 1381 agccgggtgg cggtgtgtgc ggtgctctgt ataaaaaatt tccggaaagt tttgacctgc 1441 agccgattga agtgggtaaa gcacgtctgg ttaaaggcgc agccaaacat attattcatg 1501 ccgtgggtcc gaattttaat aaggtgagtg aagttgaagg cgataaacag ctggccgaag 1561 catacgaaag cattgcaaaa attgttaatg acaacaacta caagagcgtt gcaattccgc 1621 tgctgagtac cggtattttt agcggcaata aggatcgcct gacccagagc ctgaatcatc 1681 tgctgaccgc cctggatacc accgatgccg atgttgccat ctattgccgc gataaaaaat 1741 gggaaatgac cctgaaagaa gccgtggcac gccgcgaagc agttgaagaa atttgtatta 1801 gcgatgatag tagtgttacc gaaccggatg cagaactggt gcgcgttcat ccgaaaagca 1861 gtctggccgg ccgtaaaggt tatagcacca gtgatggtaa aacctttagc tatctggaag 1921 gcaccaaatt tcatcaggcc gcaaaagata ttgcagaaat taatgcaatg tggccggtgg 1981 caactgaagc aaatgaacag gtttgcatgt atattctggg tgaaagcatg agcagcattc 2041 gtagcaaatg tccggtggaa gaaagcgaag ccagtacccc gccgagcacc ctgccgtgtc 2101 tgtgcattca tgccatgacc ccggaacgtg tgcagcgtct gaaagcaagc cgcccggaac 2161 agattaccgt gtgcagcagc tttccgctgc cgaaatatcg tattaccggc gttcagaaaa 2221 ttcagtgtag ccagccgatt ctgtttagtc cgaaagttcc ggcctatatt catccgcgta 2281 aatatctggt ggaaaccccg ccggtggatg aaaccccgga accgagcgcc gaaaatcaga 2341 gcaccgaagg tacaccggaa cagccgccgc tgattaccga agatgaaacc cgtacccgca 2401 ccccggaacc tattattatt gaagaagaag aagaggatag cattagcctg ctgagtgatg 2461 gcccgaccca tcaggtgctg caggttgaag ccgatattca tggtccgccg agtgtgagta 2521 gtagcagctg gagtattccg catgccagcg attttgatgt tgatagtctg agtattctgg 2581 ataccctgga aggtgccagc gttaccagcg gtgccaccag cgcagaaacc aatagctatt 2641 ttgccaaaag tatggaattt ctggcccgcc cggttccggc cccgagaacc gtgtttcgta 2701 atccgccgca tccggccccg cgtacccgta cccctagctt agccccgagc cgtgcctgta 2761 gtcgtaccag cctggttagt accccgcctg gtgttaatcg cgtgattacc cgtgaagaac 2821 tggaagccct gaccccgagt cgtaccccga gtcgcagtgt tagccgcacc agtctggtta 2881 gcaatccgcc gggcgttaat cgcgttatta cccgtgagga atttgaagca tttgttgcac 2941 agcagcagta aggatccacc ggatctagat aactgatcat aatcagccat accacatttg 3001 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa 3061 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca 3121 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 3181 ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc 3241 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc 3301 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag 3361 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc 3421 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa 3481 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg 3541 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt 3601 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc 3661 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 3721 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 3781 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt 3841 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 3901 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 3961 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 4021 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 4081 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 4141 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa 4201 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg 4261 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc 4321 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca 4381 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc 4441 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca 4501 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat 4561 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca 4621 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg 4681 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc 4741 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct 4801 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct 4861 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac 4921 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc 4981 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag 5041 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 5101 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg 5161 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa 5221 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt 5281 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg 5341 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca 5401 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt 5461 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 5521 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 5581 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 5641 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 5701 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag 5761 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 5821 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 5881 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 5941 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 6001 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 6061 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 6121 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 6181 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 6241 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat 6301 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt cc //
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